Ph.D: Jawaharlal Nehru University, New Delhi
BSc (Hons) & MSc: Life Sciences, Visva Bharati University, Santiniketan
ICSE & ISC: St. Joseph's Academy, Dehra Dun
a) Molecular and Cellular Endocrinology
b) Nuclear Receptors in Health and Disease
c) Endocrine-related cancers (prostate and breast cancers)
Proctor, Jawaharlal Nehru University, 2011-2013
Professor at the ‘Special Center for Molecular Medicine’, Jawaharlal Nehru University, New Delhi, India. 2011 onwards
Chairperson of ‘Special Center for Molecular Medicine’, 2007 to 2009.
Associate Professor at the ‘Special Center for Molecular Medicine’, Jawaharlal Nehru University, New Delhi, India. 2005-2011
Assistant Professor at the ‘Special Center for Molecular Medicine’, Jawaharlal Nehru University, New Delhi, India. 2001 to 2005.
NIH Visiting Research Fellowship at the University of Texas Health Science Center (UTHSCSA) to work on `Androgen Receptor gene expression and Ageing` 1998-2001.
INSERM International Fellow, Paris, France, 1995-1997.
Feinberg fellow of The Weizmann Institute of Science, Israel, 1993-1995
a) Gold medal oration award by the ‘Society of Reproductive Biology and Comparative Endocrinology’ (SRBCE) for significant contribution in the area of Molecular and Cellular Endocrinology, 2011.
b) Elected Fellow of the National Academy of Sciences (NASI), India, 2010.
c) Awarded FRE (Fellowship in Reproduction and Endocrinology) by Society of Reproductive Biology and Comparative Endocrinology, 2009
d) Awarded French Medical Research Fellowship by ‘Foundation pour la Recherche Medicale’ 1997-1998.
e) Awarded INSERM International Fellowship and French Medical Research Fellowship by ‘Foundation pour la Recherche Medicale’, France, 1995-1997.
f) Awarded Feinberg fellowship of The Weizmann Institute of Science, Israel, 1993-1995. Project: ‘Mechanism of a novel MSH-regulated signal transduction pathway in melanoma cells’.
g) Recipient of S.R.V. Rao award to Young Scientist by Indian Society of Cell Biology, 1992.
Following papers have been published with international collaborations in USA, France and Japan
Biswas A, Pasquel D, Tyagi RK, Mani S (2011) Acetylation of Pregnane X Receptor protein determines selective function independent of ligand activation. Biochem Biophys Res Commun. 406:371-376.
Amazit L, Roseau A, Ali-Khan1 J, Chauchereau A, Tyagi RK, Loosfelt H, Leclerc P, Lombes M, Guiochon-Mantel A (2011). Ligand-dependent Degradation of SRC-1 is Pivotal for Progesterone Receptor Transcriptional Activity. Molecular Endocrinology 25:394-408.
Pondugula SR, Brimer-Cline C, Wu J, Schuetz EG, Tyagi RK and Chen T (2009) A phosphomimetric mutation at threonine-57 abolishes transactivation activity and alters nuclear localization pattern of human pregnane X receptor. Drug Metabolism and Disposition 37:719-730.
Kumar S, Chaturvedi NK, Nishi M, Kawata M and Tyagi RK (2004) Shuttling components of nuclear import machinery, involved in nuclear translocation of steroid receptors, exit nucleus via exportin-1 / CRM-1 independent pathway. Biochimica et Biophysica Acta - Molecular Cell Research 1691: 73-77.
Google Scholar profile of RK Tyagi
Kumar S, Chaturvedi NK, Kumar S and Tyagi RK (2008) Agonist-mediated docking of androgen receptor onto the mitotic chromatin platform discriminates intrinsic mode of action of prostate cancer drugs. Biochimica et Biophysica Acta -Molecular Cell Research 1783:59-73. (Impact factor 5.2)
Saradhi M, Krishna B, Mukhopadhyay G and Tyagi RK (2005) Purification of full-length human Pregnane and Xenobiotic Receptor: polyclonal antibody preparation for immunological characterization. Cell Research 15: 785-795. (Impact factor 12.0)
Tyagi RK, Lavrovsky Y, Ahn CS, Song SC, Chatterjee B and Roy AK (2000) Dynamics of intracellular movement and nucleocytoplasmic recycling of the ligand-activated androgen receptor in living cells. Molecular Endocrinology 14 : 1162-1174 (Journal’s cover article). (Impact factor 4.7; article citations 250+)
Tyagi RK, Amazit L, Lescop P, Milgrom E and Guiochon-Mantel A (1998) Mechanisms of progesterone receptor export from the nuclei: role of nuclear localization signal, nuclear export signal and Ran GTP. Molecular Endocrinology, 12 : 1684-1695. ((Impact factor 4.7; article citations 80+)
Tyagi RK, Azrad A, Degani H and Salomon Y, (1996) Simultaneous extraction of cellular lipids and water-soluble metabolites: evaluation by NMR spectroscopy. Magnetic Resonance in Medicine, 35 : 194-200. (Impact factor 4.0; article citations 125+)
Kumar S and Tyagi RK (2012) Androgen receptor association with mitotic chromatin - analysis with introduced deletions and disease-inflicting mutations. FEBS Journal 279:4598-4614. (journal cover article)
Kumar S, Jaiswal B, Kumar S, Negi S and Tyagi RK (2010) Cross-talk between Androgen Receptor and Pregnane & Xenobiotic Receptor reveals existence of a novel modulatory action of antiandrogenic drugs. Biochemical Pharmacology 80: 964-976.
Chaturvedi NK, Kumar S, Negi S, Tyagi RK (2010) Endocrine disruptors provoke differential modulatory responses on Androgen Receptor and Pregnane & Xenobiotic Receptor: potential implications in metabolic disorders. Molecular and Cellular Biochemistry 345:291-308.
Tyagi RK, Negi S, Kumari S, Saradhi M and Mukhopadhyay G. Stable integration of a xenobiotic receptor promoter-reporter DNA construct into a human liver cell line for evaluating the drugs by high throughput screening. Indian Patent filed through IPM cell at JNU, New Delhi.
Indian Patent Application. No. 464/DEL/2010.
International (PCT) patent application number PCT/IN2011/000127 filed on 01.03.2011.
Publication no.: WO/2011/108003
The present claim relates to generation of a liver cell line that is stably integrated with a promoter-reporter DNA construct and has utility in analyzing those prescription drugs, herbal drug ingredients and other xenobiotics/factors that have modulatory effects on cellular levels of PXR protein i.e. up regulation or down regulation of PXR.