Structural Biology

 
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Publications in Peer-Reviewed Journals


  1. Structural insights into the interaction of helicase and primase in Mycobacterium tuberculosis Dhakaram Pangeni Sharma; Ramachandran Vijayan; Syed Arif Abdul Rehman; Samudrala Gourinath Biochemical Journal Nov 15, 2018; DOI: https://doi.org/10.1042/BCJ20180673

  2. Babuta M, Kumar S, Gourinath S, Bhattacharya S, Bhattacharya A. (2018) Calcium-binding protein EhCaBP3 is recruited to the phagocytic complex of Entamoeba histolytica by interacting with Arp2/3 complex subunit 2. Cell Microbiol. :e12942.

  3. Chadha S, Vijayan R, Gupta S, Munde M, Gourinath S, Madhubala R (2018) Genetic manipulation of Leishmania donovani threonyl tRNA synthetase facilitates its exploration as a potential therapeutic target. PLoS Negl Trop Dis.;12(6):e0006575.

  4. Singh KP, Anwar S, Zaidi A, Singh K, Das P, Gourinath S, Ali V. (2018) LdIscU is a [2Fe-2S] scaffold protein which interacts with LdIscS and its expression is modulated by Fe-S proteins in Leishmania donovani. Int J Biol Macromol. 116:1128-1145

  5. Pandey P, Verma V, Dhar SK, Gourinath S. (2018) Screening of E. coli β-clamp Inhibitors Revealed that Few Inhibit Helicobacter pylori More Effectively: Structural and Functional Characterization. Antibiotics;7(1).E5.

  6. Eniyan K, Dharavath S, Vijayan R, Bajpai U, Gourinath S. (2018) Crystal structure of UDP-N-acetylglucosamine-enolpyruvate reductase (MurB) from Mycobacterium tuberculosis. Biochim Biophys Acta. 1866(3):397-406.

  7. Devi S, Abdul Rehman SA, Tarique KF, Gourinath S. (2017) Structural characterization and functional analysis of cystathionine β-synthase: an enzyme involved in the reverse transsulfuration pathway of Bacillus anthracis. FEBS J. 284(22):3862-3880.

  8. Srivastava VK, Yadav R, Watanabe N, Tomar P, Mukherjee M, Gourinath S, Nakada-Tsukui K, Nozaki T, Datta S. (2017) Structural and thermodynamic characterization of metal binding in Vps29 from Entamoeba histolytica: implication in retromer function. Mol Microbiol. Sep 12. doi: 10.1111/mmi.13836. [Epub ahead of print]

  9. Preeti Pandey, Verma V, Gunjan G, Nilima kumara, Dhar SK and S. Gourinath. (2017) Targeting the β-clamp in Helicobacter pylori with FDA-approved drugs reveals micromolar inhibition by diflunisal. FEBS letters. 591(15):2311-2322.

  10. Gunajna Gautam, Abdul rehman SA, Pandey P, Gourinath, S. (2017) Crystal structure of the PEG-bound SH3 domain of myosin IB from Entamoeba histolytica reveals its mode of ligand recognition. Acta Cryst. (2017). D73, 672–682

  11. Verma D, Murmu A, Gourinath S, Bhattacharya A, Chary KVR. (2017) Structure of Ca2+-binding protein-6 from Entamoeba histolytica and its involvement in trophozoite proliferation regulation. PLoS Pathogens. 13(5):e1006332.

  12. S Dharavath, I Raj, S Gourinath (2017) Structure-based mutational studies of O-Acetylserine Sulfhydrylase reveal the reason for the loss of cysteine synthase complex formation in Brucella abortus. Biochemical J. 474, 1221-1239.

  13. Mazumder M, Ponnan P, Das U, Gourinath S, Khan HA, Yang J, Sakharkar MK. (20017) Investigations on Binding Pattern of Kinase Inhibitors with PPARγ: Molecular Docking, Molecular Dynamic Simulations, and Free Energy Calculation Studies. PPAR Res. 2017:6397836

  14. Preeti Pandey, Khaza Faisal Tarique, Mohit Mazumder, Syed Arif Abdul Rehman, Nilima kumari and S. Gourinath. (2016) Structural insight into β-Clamp binding site in DNA Ligase of Helicobacter pylori. Scientific Reports. 6:31181

  15. Anwar T & Gourinath S. (2016) Deep Insight into the Phosphatomes of Parasitic Protozoa and a Web Resource ProtozPhosDB. PLoS One. 11, e0167594.

  16. Ansari, MF., Siddiqui, SM., Ahmad, K., Avecilla, F., Sudhaker Dharavath, S Gourinath, Amir Azam (2016) Synthesis, antiamoebic and molecular docking studies of furan-thiazolidinone hybrids. Eur. J. of Medicinal Chem. 124, 393-406.

  17. Sanjeev Kumar, Mohit Mazumder, Nisha Gupta, Sudip Chattopadhyay and S. Gourinath. (2016) Crystal structure of Arabidopsis thaliana calmodulin7 and insight into its mode of DNA binding. FEBS letters. 590(17):3029-39. doi: 10.1002/1873-3468.12349.

  18. Singh RK., M. Mazumder, Sharma B., Gourinath, S. (2016) Structural investigation and inhibitory response of halide on phosphoserine aminotransferase from Trichomonas vaginalis. BBA (Gen). 1860(7):1508-18.

  19. Tarique, KF., Abdul Rehman, SA., Devi, S., Priya Tomar and Gourinath, S. (2016) Structural and functional insight into the stationary-phase survival protein SurE, an important virulence factor of Brucella abortus. Acta Crystallographica Section F: Structural Biology Communications. 72: 386-396.

  20. Mohit Mazumder and S. Gourinath (2016) Structure-based design of inhibitors of the crucial cysteine biosynthetic pathway enzyme O-acetyl serine sulfhydrylase. Current Topics in Medicinal Chemistry. 16(9):948-59.

  21. Mansuri MS, Babuta M, Ali MS, Bharadwaj R, Jhingan GD, Gourinath S, Bhattacharya S, Bhattacharya A. (2016) Autophosphorylation at Thr279 of Entamoeba histolytica atypical kinase EhAK1 is required for activity and regulation of erythrophagocytosis. Sci Rep. 6:16969.

  22. Rana M, Devi S, Gourinath S, Goswami R, Tyagi RK. (2016) A comprehensive analysis and functional characterization of naturally occurring non-synonymous variants of nuclear receptor PXR. Biochim Biophys Acta-GRM. 2016 Mar 4. pii: S1874-9399(16)30040-2.

  23. M. Gupta, M. Mazumder, K. Dhatchinamoorthy, M. Nongkhlaw, D. Thangminlen Haokip, S Gourinath, S. S. Komath and R. Muthuswami (2015) Ligand-induced conformation changes drive ATP hydrolysis and function in smarcal1. FEBS Journal. 282(19):3841-59.

  24. Verma S, Kumar S, Gupta VP, Gourinath S, Bhatnagar S, Bhatnagar R (2015) Structural basis of Bacillus anthracis MoxXT disruption and the modulation of MoxT ribonuclease activity by rationally designed peptides. J Biomol Struct Dyn. 33(3):606-24.

  25. Sanjeev Kumar, Saima Aslam, Mohit Mazumder, Pradeep Dahiya, Aruna Murmu, Babu A. Manjasetty, Rana Zaidi, Alok Bhattacharya and S. Gourinath (2014) Crystal Structure of Calcium Binding Protein-5 from Entamoeba histolytica and its involvement in initiation of phagocytosis of human erythrocytes. PLoS Pathogens. 10(12):e1004532.

  26. Tarique KF, Rehman SAA, Betzel Ch and S. Gourinath (2014) Structure-based identification of inositol polyphosphate 1 - phosphatase from Entamoeba histolytica. Acta Cryst D70, 3023-33.

  27. Singh RK, Raj I, Pujari R, Gourinath S. (2014) Crystal Structures and Kinetics of Type III 3-phosphoglycerate dehydrogenase reveal Catalysis by Lysine. FEBS J. 281(24):5498-512.

  28. Kumar N, Somlata, Mazumder M, Dutta P, Maiti S, and Gourinath S (2014). EhCoactosin stabilizes actin filaments in the protist parasite Entamoeba histolytica. PLoS Pathogens. 10(9):e1004362.

  29. Tarique FA., Abdulrehman, S. A, and Gourinath S (2014) Structural elucidation of a dual active PAP Phosphatase1 of Entamoeba histolytica: capable of hydrolysing both 3’adenosine5’-phosphate and inositol 1,4-bisphosphate. Acta. Crystallogr. D 70:2019-31.

  30. Kumar S, Kumar N, Alam N and Gourinath S. (2014) Crystal structure of serine acetyl transferase from Brucella abortus and its complex with coenzyme A. Biochim Biophys Acta. 1844(10):1741-1748.

  31. Mazumder M, Padhan N, Bhattacharya A and Gourinath S (2014) Prediction and analysis of canonical EF hand loop and qualitative estimation of Ca2+ binding affinity. PLoS One. 9(4):e96202.

  32. Das U, Pogenberg V, Subhramanyam UKT, Wilmanns M, Gourinath S, A Srinivasan. (2014) Crystal structure of the VapBC-15 complex from Mycobacterium tuberculosis reveals a two-metal ion dependent PIN-domain ribonuclease and a variable mode of toxin–antitoxin assembly. J. Structural Biology. 188: 249-258.

  33. George B, Ruhel R, Mazumdar M, Sharma VK, Jain SK, Gourinath S, Chakraborty S. (2014) Mutational analysis of the helicase domain of a replication initiator protein reveals critical roles of Lys 272 of B' motif and Lys 289 of β-hairpin loop in geminivirus replication. J Gen Virol. 95 :1591-602.

  34. Singh, N. K, Hasan SS, Kumar J, Raj I, Pathan AA, Parmar A, Shakil S, Gourinath S, Madamwar D. (2014) Crystal Structure and Interaction of Phycocyanin with β-Secretase: A Putative Therapy for Alzheimer's Disease. CNS Neurol Disord Drug Targets. 13 :691-698.

  35. Khan, J. M, Abdulrehman, S. A, Zaidi. F. K, Gourinath. S, and Khan R. H. (2014) Hydrophobicity alone can not trigger aggregation in protonated mammalian serum albumins. Phys Chem Chem Phys. 16:5150-61.

  36. Anwar. T and Gourinath S. (2013) Analysis of the Protein Phosphotome of Entamoeba histolytica Reveals an Intricate Phosphorylation Network. PLoS One. 8(11):e78714.

  37. Das U, Kumar N, Gourinath S, Srinivasan A. (2013) Preliminary crystallographic analysis of recombinant VapBC-15 toxin-antitoxin complex from Mycobacterium tuberculosis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 69:1242-5.

  38. Qadeer A, Ahmad E, Zaman M, Khan MW, Khan JM, Rabbani G, Tarique KF, Sharma G, Gourinath S, Nadeem S, Badr G, Khan RH. (2013) Concentration-dependent antagonistic persuasion of SDS and naphthalene derivatives on the fibrillation of stem bromelain. Arch Biochem Biophys. 540:101-16.

  39. Raj, I., Mazumder, M., Gourinath, S. (2013) Molecular basis of ligand recognition by OASS from E. histolytica: Insights from structural and molecular dynamics simulation studies. BBA (General subjects) 1830: 4573-83.

  40. Rehman, S. A. A., Verma, A., Mazumder, M., Dhar, S. K., Gourinath, S., (2013) Crystal structure and mode of helicase binding of the C-terminal domain of primase from Helicobacter pylori. J. Bacteriology, 195(12):2826-38.

  41. Kumar, S., Mazumder, M., Gourinath, S. (2013) Single residue mutation in EhSAT3 active site assists in partial regaining of feedback inhibition by cysteine. PLoS ONE. 8(2):e55932.

  42. Sanjeev Kumar, S., Zaidi, R. and Gourinath, S. (2012) Cloning, purification, crystallization and preliminary crystallographic study of calcium-binding protein 5 from Entamoeba histolytica. Acta. Crystllogr. F68. 1542-1544.

  43. Kumar S, Ahmad E, Kumar S, Khan RH, Gourinath, S. (2012) Flexibility of EF-hand motifs: structural and thermodynamic studies of Calcium Binding Protein-1 from Entamoeba histolytica with Pb2+, Ba2+, and Sr2+. BMC Biophys. 5, 15.

  44. Isha Raj, Sudhir Kumar and Gourinath, S. (2012). The narrow active site cleft of O-acetyl serine sulfhydrylase from Leishmania donovani allows complex formation with serine acetyl transferases having a range of C-terminal sequences. Acta. Crystallogr. D68, 909-19.

  45. Ahmad M. F., Yadav B., Kumar P., Puri A., Mazumder M., Ali A., Gourinath. S., Muthuswami R., and Komath S. S. (2012). The GPI anchor signal sequence dictates the folding and functionality of the ALS5 adhesin from Candida albicans. PLoS ONE (7), 4, e35305.

  46. Isha Nagpal, Isha Raj, Naidu Subbarao, S. Gourinath (2012). Virtual Screening, Identification and in vitro testing of novel inhibitors of O-Acetyl-L-Serine Sulfhydrylase of Entamoeba histolytica. PLoS ONE, 7(2):e30305.

  47. Javed M. Khan, Atiyatul Qadeer, Sumit K. Chaturvedi, Ejaz Ahmad, Syed Arif A. Rehman, S. Gourinath, Rizwan H. Khan (2012). SDS Can Be Utilized as an Amyloid Inducer: A Case Study on Diverse Proteins. PLoS ONE. (7),1, e29694.

  48. Sudhir Kumar, Isha Raj, Isha Nagpal, N. Subbarao and S. Gourinath (2011). Structural and biochemical studies of Serine Acetyltransferase reveal why the parasite Entamoeba histolytica cannot form Cysteine Synthase complex. J. Biol. Chem. 286 (14), 12533-41.

  49. Shivesh Kumar, Ejaz Ahmad, M. Shahid Mansuri, Sanjeev Kumar, Ruchi Jain, Rizwan Hasan Khan, S. Gourinath (2010). Crystal structure and trimer-monomer transition of N-terminal domain of EhCaBP1 from Entamoeba histolytica. Biophys. J. (98). 2933-2942.

  50. Kashav, T., Nitharwal, R., Abdulrehman, S.A., Gabdoulkhakov, A., Saenger, W., Dhar, S.K. Gourinath, S (2009). Three-dimensional structure of N-terminal domain of DnaB Helicase and Helicase-Primase interactions in Helicobacter pylori. PLoS ONE. (10):e7515.

  51. Ranjan, R., Ahmed, A., Gourinath, S., Sharma, P. (2009). Dissection of mechanisms involved in the regulation of plasmodium falciparum calcium dependent protein kinase 4 (PfCDPK4). J. Biol. Chem. 284(22):15267-76.

  52. Jain, R., Kumar, S., Gourinath, S., Bhattacharya, S., Bhattacharya, A. (2009). N- and C-terminal domains of the calcium binding protein EhCaBP1 of the parasite entamoeba histolytica display distinct functions. PLoS ONE. 4(4):e5269.

  53. Krishna Ch, Kumar M, Kumar S, Jain S, Alam N, Gourinath S. (2008) Crystal structure of native O-acetyl-serine sulfhydrylase from Entamoeba histolytica and its complex with cysteine: Structural evidence for cysteine binding and lack of interactions with Serine acetyl transferase. Proteins.72 ,1222-1232

  54. Brown, J. H., Yuting, Y., Reshetnikova L., Gourinath, S., Suveges, D., Kardos, J., Hobor, F., Reutzel, R., Nyitray L. and Cohen, C. (2008). An Unstable Head–Rod Junction May Promote Folding into the Compact Off-State Conformation of Regulated Myosins. J. Mol. Biol. 325, 1434-1443.

  55. Kumar, S., Pradhan, N., Alam, N., and Gourinath S. (2007) Crystal structure of Calcium binding protein-1 from Entamoeba histolytica : novel arrangement of EF hand motifs. Proteins. 68, 990-998.

  56. Krishna, C., Jain, R., Kashav, T., Wadhwa, D., Alam, N., and Gourinath S. (2007) Crystallization and preliminary crystallographic analysis of cysteine synthase from Entamoeba histolytica. Acta. Cryst. F63, 512 – 515.

  57. Yuting, Y., Gourinath, S#., Kovács M, Nyitray L, Reutzel R, Himmel DM, O'Neall-Hennessey E, Reshetnikova L, Szent-Györgyi AG, Brown JH, Cohen C. (2007) Rigor-like states in muscle myosins reveal key mechanical elements in the transduction pathways of this allosteric motor. Structure, 15, 553 – 564. ( equally contributing first author). Appeared on cover page with a preview on this article.

  58. Nitharwal, R. G., Paul, S,. Dar, A., Choudhury, N. R., Soni, R. K., Prusty, D., Sinha, S., Kashav, T., Mukhopadhyay, G., Chaudhuri, T. K., Gourinath, S., and Dhar, S. K. (2007). The domain structure of Helicobacter pylori DnaB helicase: the N-terminal domain can be dispensable for helicase activity whereas the extreme C-terminal region is essential for its function. Nucleic. Acid. Res. 35, 2861 – 2874.

  59. Mehra, P., Biswas, A, K., Gupta, A., Gourinath, S., Chitnis, C. E. & Dhar, S. K. (2005). Expression and characterization of human malaria parasite Plasmodium falciparum origin recognition complex subunit 1. BBRC, 337, 955-66.

  60. Gourinath, S#., Pradhan, N., Alam, N. & Bhattacharya, A., (2005) Crystallization and preliminary crystallographic analysis of Calcium binding protein-2 from Entamoeba histolytica and it’s complexes with Strontium and IQ1 motif of myosin V. Acta. Cryst. F61, 417-420.

  61. Garima, S., Gourinath, S., Sharma, S., Paramasivam, M., Srinivasan, A. & Singh, T. P. (2005) Specific mutations in krait PLA2 lead to dimerization: Crystal structure of krait PLA2 at 2.1 Å resolution. J. Struct. Biol. 147, 264-72.

  62. Garima, S., Gourinath, S., Saravanan, K., Sharma, S., Bhanumathi, S., Betzel, Ch., Srinivasan, A. & Singh, T. P. (2005) Sequence induced trimerization of krait PLA2 : Structure of the trimeric isoform form of PLA2 from common krait (Bungarus caeruleus) at 2.5 Å resolution. Acta. Cryst. F61, 8-13.

  63. Gourinath S, Risal D., Himmel, D. M., Szent-Gyorgyi, A. G. & Cohen, C. (2004) Myosin subfragment1 structures reveal a partially bound nucleotide and a complex salt bridge that helps couple nucleotide and actin binding. Porc. Natl. Acad. Sci. USA. 101, 8930-8935.

  64. Alam, N, Stieglitz, K. A., Caban, M. D., Gourinath, S., Tsuruta, H., Kantrowitz, E. R. (2004) 240's loop interactions stabilize the T state of Escherichia coli aspartate transcarbamoylase. J Biol Chem. 279, 23302-10.

  65. Gourinath, S., Himmel, D. M., Brown, J., Reshetnikova, L., Szent-Gyorgyi, A. G. & Cohen, C. (2003). Crystal structure of the scallop myosin S1 in pre-powerstroke conformation to 2.6 Å resolution: flexibility and function in the head. Structure, 11, 1621-1627.

  66. Himmel, D. M., Gourinath, S., Reshetnikova, L., Shen, Y., Szent-Gyorgyi, A. G. & Cohen, C. (2002). Crystallographic findings on the internally uncoupled and near-rigor states of myosin: Further insights into the mechanics of the motor. Proc. Natl. Acad. Sci.USA. 99, 12645-12650.

  67. Singh, R. K., Gourinath, S., Sharma, S., Roy, I., Gupta, M. N., Betzel, Ch. and Singh. T. P. (2001) Enhancement of enzyme activity through three - phase partitioning: Crystal structure of a modified serine proteinase at 1.5 Å resolution. Prot. Engg. 14, 307-313.

  68. Garima, S., Gourinath, S., Sharma, S., Paramasivam, M., Srinivasan, A. & Singh, T. P. (2001) Sequence and crystal structure determination of a basic phospholipase A2 from common krait (Bungarus caeruleus) at 2.4 Å resolution: identification and characterization of its pharmacological sites. J. Mol. Biol. 307, 1049-59.

  69. Alam, N., Gourinath, S., Dey, S., Srinivasan, A. & Singh, T. P. (2001) Substrate-Inhibitor Interactions in the Kinetics of alpha-Amylase Inhibition by Ragi alpha-amylase/Trypsin Inhibitor (RATI) and Its Various N-Terminal Fragments. Biochemistry. 40, 4229-33.

  70. Gourinath, S., Degenhardt, M., Eschenburg, K., Moore, L. J. Delucas, L. J., Betzel Ch & Singh, T. P. (2001) Structure of a partially modified Proteinase K by mercury. Ind. J. Biochem. Biophy. 38, 298-302.

  71. Betzel, C., Gourinath, S., Kumar, P., Kaur, P., Perbandt, M., Eschenburg, S. & Singh T.P. (2001) Structure of a serine protease proteinase K from Tritirachium album limber at 0.98 Å resolution. Biochemistry. 40, 3080-3088.

  72. Gourinath, S., Alam, N., Srinivasan, A. & Singh, T. P. (2000) Crystal structure of the bifunctional inhibitor of trypsin and alpha-amylase from ragi seeds at 2.2 Å resolution. Acta. Cryst. D56, 287-293.

  73. Gourinath, S., Srinivasan, A. & Singh, T.P. (1999) Crystal structure of the bifunctional inhibitor of trypsin and alpha-amylase from ragi seeds at 2.9 Å resolution. Acta Cryst D55, 25-30.


    74. Book Chapter

    1. Isha Raj, Sudhir Kumar, Mohit Mazumder and S. Gourinath. Structural biology of de novo cysteine biosynthetic pathway enzymes. by SPRINGER. Edited by Tomo Nozaki and Alok Bhattacharya.


    Refreed Papers in Published Conference Proceedings

    1. Structural Basis of an Essential Interaction between DnaG and DnaB in Mycobacterial tuberculosis Dhakaram P.Sharma, Ramachandran Vijayan, Arif Abdul Rehman, Samudrala Gourinath 1105-PosB14DOI:https://doi.org/10.1016/j.bpj.2017.11.1227

    2. Tale of the two domains in twinfilin: Deciphering phagocytosis through actin dynamics Pragyan Parimita Rath, Nitesh Kumar, Mrigya Babuta, Alok Bhattacharya and S Gourinath Published Online:20 Apr 2018Abstract Number:542.30

    3. Nitesh Kumar, Sankar Maiti, Saima Aslam, Somalata and S. Gourinath (2012). Structural and functional studies of Coactosin from Entamoeba histolytica.EMBO Global Exchange Lecture Course & Symposium on “Amoebiasis: Exploring the Biology and the Pathogenesis of Entamoeba” held during 4-7th , March 2012 at Khajuraho, India.

    4. Sanjeev Kumar, Rana Zaidi  and S.Gourinath (2012). Crystal Structure of CaBP5 from Entamoeba histolytica at EMBO Global Exchange Lecture Course & Symposium on “Amoebiasis: Exploring the Biology and the Pathogenesis of Entamoeba” held during 4-7th , March 2012 at Khajuraho, India.

    5. Sudhir Kumar, Isha Raj, Isha Nagpal and S. Gourinath "Structural and Biochemical Studies of Cysteine Biosynthetic Enzymes Reveal Why the Parasite Entamoeba histolytica Cannot Form a Cysteine Synthase Complex" at Hamburg school for Structure and Dynamics in infection, International symposium n structural and infection biology, 1-2nd July 2011.

    6. S. Arif Abdulrehman, Tara Kashav, Ramgopal Nitharwal, Suman Kumar Dhar,and Gourinath .S. "Helicase-Primase interactions and their structural studies in Helicobacter pylori" at 55th Biophysical society held on 5th March – 9th March 2011 at Baltimore, Maryland, USA.

    7. Sudhir Kumar and Gourinath, S. "Regulation of cysteine biosynthetic pathway in the light of crystal structure of Entamoeba histolytica serine acetyltransferase 1" at 7th Asian Biophysics Association (ABA) Symposium & Annual Meeting of the Indian Biophysical Society (IBS) held on 30th Jan - 2nd Feb 2011 at India Habitat Center, New Delhi, India.

    8. S. Arif Abdulrehman, Tara Kashav, Ramgopal Nitharwal, Suman Kumar Dhar,and Gourinath .S. "Structural studies of DNA replication initiation complex proteins Primase and Helicase from Helicobacter pylori" at 7th Asian Biophysics Association (ABA) Symposium & Annual Meeting of the Indian Biophysical Society (IBS) held on 30th Jan - 2nd Feb 2011 at India Habitat Center, New Delhi, India.

    9. Isha Raj, Isha Nagpal and Gourinath S. "Two mutations at the C-terminal end of EhSAT could restore EhOASS-EhSAT interactions" at 7th Asian Biophysics Association (ABA) Symposium & Annual Meeting of the Indian Biophysical Society (IBS) held on 30th Jan - 2nd Feb 2011 at India Habitat Center, New Delhi, India.

    10. Sanjeev Kumar, Shivesh Kumar, Ejaz Ahmed, M. Shahid Mansuri, Rana Zaidi, Rizvan Hasan Khan and Gourinath, S. "Calcium binding protein 1 of Entamoeba histolyitca: a dual regulator" at 7th Asian Biophysics Association (ABA) Symposium & Annual Meeting of the Indian Biophysical Society (IBS) held on 30th Jan - 2nd Feb 2011 at India Habitat Center, New Delhi, India.

    11. Nitesh Kumar and Gourinath, S. "Three dimensional structure of Coactosin from Entamoeba histolytica at 1.4 Å resolution" at 7th Asian Biophysics Association (ABA) Symposium & Annual Meeting of the Indian Biophysical Society (IBS) held on 30th Jan - 2nd Feb 2011 at India Habitat Center, New Delhi, India.

    12. Isha Nagpal and Gourinath, S. "In-silico screening of inhibitors against OASS and SAT of Entamoeba histolytica cysteine biosynthetic pathway" at 7th Asian Biophysics Association (ABA) Symposium & Annual Meeting of the Indian Biophysical Society (IBS) held on 30th Jan - 2nd Feb 2011 at India Habitat Center, New Delhi, India.

    13. Sushant Kumar, S. Arif Abdulrehman and S. Gourinath. "Role of linker region in hexamerizzation of DnaB replicative helicase in Eubacteria" at 7th Asian Biophysics Association (ABA) Symposium & Annual Meeting of the Indian Biophysical Society (IBS) held on 30th Jan - 2nd Feb 2011 at India Habitat Center, New Delhi, India.

    14. S. Arif Abdulrehman, Tara Kashav, Ramgopal Nitharwal, Suman Kumar Dhar, and S. Gourinath. "Helicase-primase interactions and their structural studies in Helicobactor pylori" 39th National seminar on crystallography on Oct 25-27, 2010 at Department of Physics and electronics, University of Jammu, Jammu Tawi.

    15. Sudhir Kumar and S. Gourinath. "Crystal structure of Entamoeba histolytica serine acetyletransferase1 and its complex with Ser and Cysteine". 39th National seminar on crystallography on Oct 25-27, 2010 at Department of Physics and electronics, University of Jammu, Jammu Tawi.

    16. S. Arif Abdulrehman, Tara Kashav, Ramgopal Nitharwal, Suman Kumar Dhar, and S. Gourinath. "Helicase-primase interactions and their structural studies in Helicobactor pylori" 4th International symposium on recent trends in macromolecular structure and function on Jan 21st-23rd Jan 2010 at University of Madras, Guiny campus, Chennai.

    17. Isha Raj, Isha Nagpal and S. Gourinath. "Investigations into the loss of EhOASS-EhSAT interactions" 4th International symposium on recent trends in macromolecular structure and function on Jan 21st-23rd Jan 2010 at University of Madras, Guiny campus, Chennai.

    18. Nitesh Kumar and S. Gourinath. "Three dimensional structure of Coactosin from Entamoeba histolytica at 1.4Å resolution." 4th International symposium on recent trends in macromolecular structure and function on Jan 21st-23rd Jan 2010 at University of Madras, Guiny campus, Chennai.

    19. Sudhir Kumar and S. Gourinath. "Crystal structure of Entamoeba histolytica serine acetyletransferase1 and its complex with cysteine." 4th International symposium on recent trends in macromolecular structure and function on Jan 21st-23rd Jan 2010 at University of Madras, Guiny campus, Chennai.

    20. Sudhir Kumar and Gourinath, S. "Crystallization and Preliminary crystallographic data of Serine acetyltranferase from Entamoeba histolytica" National Seminar on Crystallography, Mysore, Feb. 11-13, 2009.

    21. Kumar, S. and Gourinath, S. Crystal structure of calcium binding protein-1 from Entamoeba histolytica in complex with phenylalanine: Visualization of mode of target binding. EMBO world lecture course on Recent developments in Macromolecular Crystallography, at NCL, Pune, 9-14 Nov 2008.

    22. Kumar, S. and Gourinath, S. Crystal structure of calcium binding protein-1 from Entamoeba histolytica in complex with phenylalanine: Visualization of mode of target binding. International Symposium on recent trends in macro molecular structure and function, Jan 7-12th 2008, Chennai. (Got best poster award).

    23. Kumar, M., Krishna, Ch., Kumar, S., Jain, S., Alam, N. and S. Gourinath. Crystal structure of native O-acetyl-serine sulfhydrylase from Entamoeba histolytica and its complex with cysteine: Structural evidence for cysteine binding and lack of interactions with Serine acetyl transferas. International Symposium on recent trends in macro molecular structure and function, Jan 7-12th 2008, Chennai.

    24. Alam, N, Kumar, S, Pradhan N,. and Gourinath, S. "Crystal structure of a calcium binding protein-1 from Entamoeba histolytica : a novel arrangement of EF hands motifs". At National Symposium of Crystallography, January 22-24th 2007, Chennai.

    25. Tara, K, Rajaram A, Alam N, Dhar SK and Gourinath S. "N-terminal domain dictates the quaternary states of Helicobacter pylori hexameric DnaB helicase. At society of biological chemistry, 75th Annual meeting Dec 8th-11th 2006. Delhi.

    26. Kumar, S, Pradhan N,. Alam, N. and Gourinath S. "Crystal structure of a calcium binding protein-1 from Entamoeba histolytica : a novel arrangement of EF hands motifs. At society of biological chemistry, 75th Annual meeting Dec 8th-11th 2006. Delhi.

    27. Tara, K., Alam, N., Dhar, S. K. and Gourinath, S. "Structural studies of DnaB helicase from Helicobactor pylori". At Symposium on Macromolecular Crystallography. Nov 15-16, 2005. Hyderabad.*Got best poster presentation award.

    28. Gourinath, S., Srinivasan, A. and Singh, T. P. "Structure of novel bifunctional Inhibitor of trypsin and alpha -amylase from Ragi seeds. "At Cambridge healthtech Institute's Proteases Inhibitors: New therapeutics and approaches, Nov 6-7, 1996. Baltimore, Maryland.

    29. Gourinath, S., Srinivasan, A. and Singh, T. P. "Crystal structure of the bifunctional inhibitor of trypsin and alpha-amylase from Ragi seeds at 3.3 Å resolution." At XXVIII National seminar on Crystallography, september 24-26, 1997. Kottayam, Kerala.

    30. Gourinath, S., Neelima, A., Srinivasan, A., Singh, T. P. "Crystal structure of the bifunctional inhibitor of trypsin and alpha-amylase from Ragi seeds at 2.9 Å resolution." At XIII Inter National seminar on Biophysics, september 29-24, 1999. Delhi.

    31. Gourinath, S., Sharma, S., Singh, R. K., Ray, I., Gupta, M. N. and Singh, T. P. "Enhancement of enzyme activity through three - phase partitioning : Crystal structure of a modified serine proteinase at 1.5 Å resolution." At National seminar on Recent trends in Crystallography, Biophysics and Computational Biology. April, 24-26, 2000. Chennai. *Got best poster presentation award.

    32. Garima, S., Sharma, S., Gourinath, S., Paramasivam, M., Srinivasan, A. and Singh, T. P. "Structure of Phospholipase A2 from Indian common Krait (Bungarus Caeruleus) venom at 2.4 Å resolution." At National seminar on Recent trends in Crystallography, Biophysics and Computational Biology. April, 24-26, 2000. Chennai.



    Poster Presentations

    1. Poster presentation titled "Structural and functional studies of Entamoeba histolytica Serine acetyltransferases at "International Conference on Biomolecular Forms and Function, A Celebration of 50 years of Ramachandran Map" organized by Indian Institute of Sciences, Bangalore on 8-11 January, 2013. *Got First Prize by Protein Society, USA.

    2. Poster presentation titled "Structural and functional studies of Entamoeba histolytica Serine acetyltransferases; insight into the differential regulation of isoforms' at National Symposium on "Frontiers of Biophysics, Biotechnology & Bioinformatics" & 37th Annual Meeting of Indian Biophysical Society (IBS) held on 13th Jan-16th Jan, 2013 at University of Mumbai, India. *Got Prof. A.R. Gopala Ayengar Best Poster Award by Indian Biophysical Society.




Jawaharlal Nehru University, New Delhi
110067 INDIA
Phone: +91- 011 011-26704513
Email: sgourinath@mail.jnu.ac.in

Updated November 2018 @ JNU