Postdoctoral Training (Immunology): The Cleveland Clinic Ohio, USA
Doctoral (Biotechnoloy): Jadavpur University Kolkata, India
Masters’ (Microbiology): M.S. University Baroda, India
We work on early host-microbe interactions, with specific emphasis on human epithelial innate immunity and bacterial biofilms. The human epithelial surface is continuously exposed to microbes and acts as the first line of defense against infections. Arrays of Pattern Recognition Receptors on epithelium associated cells discriminate between commensal vs. pathogens microbes; and produce endogenous antibiotics like defensins against pathogens. The latter develop biofilms, which use complex Quorum signalling to generate virulence factors that can outcompete these immune determinants. The balance between these competing early host-microbe forces often determine the outcomes of infections. In this context we use novel biofilm models, innate immune arrays, transcriptomics and mass spectrometry based metabolomics to investigate these domains in collaboration with leading hospitals. As part of this we are currently part of the National Neonatal Sepsis Research Consortium (DBT), one of the largest research programs in India in collaboration with AIIMS, TSHTI, IGIB, NII and ICGEB.
2008 - present ; Assistant Professor, Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
2009-2012 - Faculty-In-Charge, Mass Spectrometry Laboratory, Advanced Instrumentation Facility, Jawaharlal Nehru University, New Delhi, India
2003-2008 (Feb.) - Faculty Scientist, Indian Institute of Technology, Kharagpur, India
1997-2002 - Postdoctoral Fellow, Department of Immunology & Department of Gastroenterology, The Cleveland Clinic Foundation, USA.
Merlin Bumpus Best Investigator Award, USA, 2001.
Prof. Sanjoy K. Bhattacharya, Bascom Palmer Eye Institute, Florida, U.S.A (Lipidomics).
National Collaborations:
The Indian Neonatal Sepsis Consortium (AIIMS-Del, NII, ICGEB, TSHTI, IGIB & IIIT).
Prof. Rakesh Lodha, Department of Pediatrics, All India Institute of Medical Sciences, New Delhi (Hospital Associated Infections).
Dr. Venkat Panchagnula, National Chemical Laboratory, Pune (Laser Desorption Ionization Mass Spectrometry).
1. Lahiri P., Dhaware D., Singh A., Panchagnula V. & Ghosh D. (2019) Quantitation of Neurotoxic Metabolites of the Kynurenine Pathway by Laser Desorption Ionization Mass Spectrometry (LDI-MS). Methods Mol Biol 1996, 113-129,
2. Pompilio A, Crocetta V, Ghosh D et al. (2016) Stenotrophomonas maltophilia phenotypic and genotypic diversity during a 10-year colonization in the lungs of a cystic fibrosis patient. Frontiers in Microbiology 2016; 7: 1551
3. Pluháček T, Lemr K, Ghosh D, Milde D, Novák J and Havlíček V (2016) Characterization of Microbial Siderophores by Mass Spectrometry. Mass spectrometry Reviews. 35: 35-47
4. Ghosh D., Salzman NH, Huttner KM, Paterson Y, Bevins CL. Protection against enteric salmonellosis in transgenic mice expressing a human intestinal defensin. Nature. 422:522-6.Commentary: Ganz T. Microbiology: Gut defence. Nature. 422:478-9.
5. Ghosh D., Porter E, Shen B, Lee SK, Wilk D, Drazba J, Yadav SP, Crabb JW, Ganz T, Bevins CL. Paneth cell trypsin is the processing enzyme for human defensin-5. Nat. Immunol. 3:583-590. Commentary: Zasloff M. Trypsin, for the defense. Nat Immunol. 2002 Jun;3(6):508-10.
Ghosh, D. (2011). Pathogenesis of Malabsorption Syndrome: Issues on Gut Flora and Innate Immunity. In: Ghoshal, U Malabsorption Syndrome in Tropics. Delhi: Elsevier. 179-204.
Ghosh, D. (2010). Probiotics and Intestinal Defensins: Augmenting the First Line of Defense in Gastrointentinal Immunity. In: Nair, G. B. and Takeda, Y. Probiotic Foods in Health and Disease. Delhi: Oxford & IBH Publishing Co. 61-74
1. Selective Detection and Analysis of Small Molecules Ghosh D., Dharware D. and Panchagnula V. Jawaharlal Nehru University, New Delhi and Central Scientific and Industrial Research (CSIR), New Delhi. (Ind) 407/DEL/2011; (EPO) EP2676287A2 ; (USPTO) 20130323849A1. Technology Ready for Transfer.
