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Rajesh Mishra

Rajesh Mishra
Rajesh Mishra
Professor
Centre/School/Special Centre
School of Biotechnology
Room No
101, SBT
Off. Phone
011-26738886
Email
rajeshmishra@mail.jnu.ac.in
Personal Webpage
https://jnu.ac.in
Qualifications

B.Sc. (Botany, Chemistry & Zoology), Ewing Christian College, University of Allahabad, India (1992)
M.Sc. (Biochemistry), G.B. Pant University of Agriculture & Technology, Pantnagar, India (1995)
Ph.D. (Biotechnology), Jawaharlal Nehru University, New Delhi, India (2002). The research work also carried out (1999-2001) under "DAAD sandwich PhD. programme" at University of Potsdam, Germany. 

Areas of Interest/Specialization

Protein Science/Protein Folding, Aggregation and Amyloid Fibril Formation

Experience

2022- Present: Professor, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India.
2015-2022:  Associate Professor, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India.
2009-2015: Assistant Professor, Centre of Biotechnology, University of Allahabad, India.
2011-2013: Visiting Researcher, Linköping University, Sweden. 
2006-2009: Research Associate, Dortmund University of Technology, Germany. 
2005-2006: Postdoctoral Fellow, Linköping University, Sweden. 
2002-2004: Postdoctoral Research Associate, The University of Tennessee, Knoxville, TN, USA 

Awards & Honours

* DAAD long term fellowship under “Sandwich Ph.D. Programme” (1999 to 2001), University of Potsdam, Germany.
* Qualified CSIR–UGC test for Junior Research Fellowship and eligibility of university lecturership (1996). 
* Qualified GATE (Graduate Aptitude Test in Engineering) 1995.
* G.B. Pant University Graduate Teaching Assistantship (1994-1995)

Best Peer Reviewed Publications
  1. Rajesh Mishra, Robert Seckler and Rajiv Bhat. Efficient refolding of aggregation prone Citrate Synthase by polyol osmolytes: How well are protein folding and stability aspects coupled? Journal of Biological Chemistry (2005). 280, 15553-15560.
  2. Neil A. Whittemore*, Rajesh Mishra*, Indu Kheterpal, Angela D. Williams, Ronald Wetzel and Engin H. Serpersu. Hydrogen-deuterium exchange mapping of Abeta 1-40 amyloid fibril secondary structure using nuclear magnetic resonance spectroscopy. Biochemistry (2005). 44, 4434-4441. (* Equal contributing authors)
  3. Rajesh Mishra, Karin Sörgjerd, Amanda Nordigården, Sofie Nyström, Yen-Chi Yu and Per Hammarström. Lysozyme amyloidogenesis is accelerated by specific nicking and fragmentation but decelerated by intact protein binding and conversion. Journal of Molecular Biology (2007). 366, 1029-1044.
  4. Rajesh Mishra, Rajiv Bhat and Robert Seckler. Chemical chaperone mediated protein folding: Stabilization of P22 tailspike folding intermediates by glycerol. Biological Chemistry (2007). 388, 797-804.
  5. Rajesh Mishra, Bruno Bulic, Daniel Sellin, Suman Jha, Herbert Waldmann, and Roland Winter. Small molecule inhibitors of islet amyloid polypeptide fibril formation. Angewandte Chemie international edition (2008). 47, 4679-4682.
  6. Rajesh Mishra and Roland Winter. Cold- and pressure-induced dissociation of protein aggregates and amyloid fibrils. Angewandte Chemie international edition (2008). 47, 6518-6521.
  7. Rajesh Mishra, Linus Olofsson, Martin Karlsson, Uno Carlsson, Ian Nicholls and Per Hammarström. Conformationally isoformic thermophilic protein with high kinetic unfolding barriers. Cellular and Molecular Life Sciences (2008).  65, 827-839.
  8. Rajesh Mishra, Daniel Sellin, Diana Radovan, Andrea Gohlke, and Roland Winter. Inhibiting the islet amyloid polypeptide fibril formation by the red wine compound resveratrol. ChemBioChem (2009). 10, 445-449.
  9. Rajesh Mishra, Matthias Geyer and Roland Winter. NMR spectroscopic investigation of early events in IAPP amyloid fibril formation.ChemBioChem (2009). 10, 1769-1772.
  10. Satish Babu Moparthi, Rikard Fristedt, Rajesh Mishra, Karin Almstadt, Martin Karlsson, Per Hammarström and Uno Carlsson Chaperone activity of Cyp18 through hydrophobic condensation that enables rescue of transient molten globule intermediates. Biochemistry (2010). 49, 11376-1145.
  11. Rajesh Mishra*, Daniel Sjölander* Per Hammarström. Spectroscopic characterization of diverse amyloid fibrils in-vitro by the fluorescent dye Nile red.  Molecular Biosystems (2011). 7, 1232-1240.
  12. Per Hammarström, Malik M. Ali, Rajesh Mishra, Belma Salagic, Pentti Tengvall, Samuel Svensson and Ingemar Lundström. An auto-catalytic surface for conformational replication of amyloid fibrils- Genesis of an amyloid world? Origins of Life and Evolution of Biospheres (2011). 41, 373-383 .
  13. Sofie Nyström, Rajesh Mishra, Simone Hornemann, Adriano Aguzzi, K.Peter R. Nilsson and Per Hammarström. Multiple substitutions of methionine 129 in human prion protein reveal its importance in the amyloid fibrillation pathway. Journal of Biological Chemistry (2012). 287, 25975-84.
Recent Peer Reviewed Journals/Books
  1. Rajesh Mishra, Matthias Elgland, Afshan Begum, Timmy Fyrner, Peter Konradsson, Sofie Nyström, and Per hammerström. Impact of N-glycosylation site variants during human PrP aggregation and fibril nucleation. BBA Proteins and Proteomics (2019). 1867, 909-921. 
  2. Aziz Ahmad, Rajesh Mishra.  Different unfolding pathways of homologous alpha amylases from Bacillus licheniformis (BLA) and Bacillus amyloliquefaciens (BAA) in GdmCl and urea. International Journal of Biological Macromolecules (2020).159, 667-674. 
  3. Rahamtullah, Rajesh Mishra. Nicking and fragmentation are responsible for α-lactalbumin amyloid fibril formation at acidic pH and elevated temperature. Protein Science (2021). 30, 1919-1934. 
  4. Aziz Ahmad, Rajesh Mishra. Differential effect of polyol and sugar osmolytes on the refolding of homologous alpha amylases: A comparative study. Biophys Chem. (2022) 281:106733. doi: 10.1016/j.bpc.2021.106733.
  5. Aziz Ahmad, Rahamtullah and Rajesh Mishra. Structural and functional adaptation in extremophilic microbial α-amylases. Biophys Rev(2022);14:499-515
  6. Lipsa Choudhury, Esha Shukla, Rajender Jena, Vishwanath Yadav, Aziz Ahmad, Rajesh Mishra and Gaurav Pandey. Staphylococcal Protein A with Engineered Cysteine: Comparison of Monomeric Content as a Critical Quality Attribute during Intracellular and Extracellular Expression Fermentation (2022); 8, 150. 
  7. Rahamtullah, Aziz Ahmad and Rajesh Mishra. Polyol and sugar osmolytes stabilize the molten globule state of α-lactalbumin and inhibit amyloid fibril formation. BBA- Proteins and Proteomics. (2022);1870:140853.
  8. Aziz Ahmad, Prachi Joshi and Rajesh Mishra. Amino acids and glycine derivatives differently affect refolding of mesophilic and thermophilic like α-amylases: implications in protein refolding and aggregation. J Biomol Struct Dyn. (2024) Mar 14:1-14. doi: 10.1080/07391102.2024.2327540.
  9. Charu Thapliyal, Rajesh Mishra. The Chaperone-Active State of HdeB at pH 4 Arises from Its Conformational Rearrangement and Enhanced Stability Instead of Surface Hydrophobicity. Biochemistry, 2024. doi: 10.1021/acs.biochem.4c00132.